Fighting Neuroblastomas with NBAT1

Children are vulnerable to extracranial solid tumours, known as neuroblastomas, at a very young age. These tumors are derived from primitive sympathetic neural precursors and they account for almost 15% of all pediatric cancer deaths (1). The most distinguishing feature of these tumors is their heterogeneous clinical behavior, which can range from spontaneous regression to highly aggressive metastatic disease. This variability in the disease outcome depends on several factors such as the age of the patient, the stage of the disease, and the genetic profile. In the last decade, we have witnessed major advances in the field of tumor biology and in drug development for treatment of various cancers including neuroblastoma. Despite these recent diagnostic and clinical advancements, the survival rate of patients with high-risk neuroblastomas still remains less than 50 per cent (2). The poor clinical outcomes in high-risk patients compel us to improve our understanding of these tumors further and to use more innovative approaches to identify candidate genes that may contribute to the disease. Our recent study published in Cancer Cell journal has employed one such approach, whereby transcriptomes of lowand high-risk neuroblastomas were sequenced to identify differentially expressed long noncoding RNAs (lncRNAs) with possible roles in disease pathogenesis (3). LncRNAs are untranslated transcripts more than 200bp in size, with known regulatory functions in various key biological processes that have been implicated in development and differentiation (4). We identified a set of 24 unannotated lncRNAs as being significantly differentially expressed in lowand high-risk neuroblastomas. In addition, we also identified differentially expressed lncRNAs that mapped to characteristic non-random chromosomal aberrations associated with high-risk neuroblastomas such as the MYCN amplified region on chromosome 2, and chromosomal deletions at 1p and 11q regions. These apparently differentially expressed lncRNA signatures might serve as biomarkers for determination of the prognosis of neuroblastomas, and their functional characterization would give major clues about the underlying biology of neuroblastoma pathogenesis. One of the most interesting findings in our transcriptome study was the functional characterization of a lncRNA LOC729177 (known as neuroblastoma associated transcript1 or NBAT1; formerly referred to as NBAT-1) that maps to a neuroblastoma hotspot on the 6p22 region. This region has been shown to harbor three neuroblastoma associated SNPs and one of them―rs6939340―maps to intron 2 of NBAT1 (5). This lncRNA has specific clinical and functional characteristics that make it a highly interesting molecule regarding neuroblastoma. NBAT1 expression analysis in large datasets of 591 tumors showed that low expression is associated with poor clinical outcomes. Moreover, NBAT1 expression can be used as an independent prognostic marker for prediction of event-free survival in neuroblastoma patients. Our study demonstrated that the lower levels of NBAT1 in high-risk patients are due to two intricate transcriptional regulatory mechanisms involving CpG methylation and genotype at the high-risk neuroblastoma-associated SNP rs6939340. Hypermethylation of the NBAT1 promoter and G/G genotype at the high-risk neuroblastoma-associated SNP were found to be significantly associated with lower Editorial